Two enzymes responsible for restricting the growth of bacte…

Biology Biotechnology Principles and Processes Single Correct MCQ

Published on: August 21, 2026

Two enzymes responsible for restricting the growth of bacteriophages in Escherichia coli were isolated. One was methylase and other was restriction endonuclease. What was the significance of methylase?

Able to cut the DNA of bacteriophage at specific sites

Able to remove the methyl group and hence prevent the action of restriction endonuclease on host DNA

Protection of host DNA from the action of restriction endonuclease by adding methyl group to one or two bases usually within the sequence recognized by restriction enzyme

Able to ligate the two cohesive ends of DNA molecule

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The correct answer is:

Methylase protects the bacterial (host) DNA by adding methyl groups to specific bases within the recognition sequence. This prevents the restriction endonuclease from cutting the host DNA, while foreign DNA (such as bacteriophage DNA) remains unprotected and is cut.

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